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Good’s Buffers (Biological buffers) refer to the group of buffers described in the research of Dr. Norman Good et al. in 1966. These buffers were selected because they display characteristics making them integral to research in biology and biochemistry.
The characteristics associated with a Good’s buffer include the following:
pKa value between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240 nm and 700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations, and limited permeability of biological membranes.
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